Expression of GABA system in Mouse Olfactory Mucosa
نویسندگان
چکیده
Our previous study showed the presence of γ-amonobutyrate (GABA) system composed of GABA synthesize enzyme glutamate decarboxylase (GAD), GABA and GABA receptors in lower airway tract epithelial cells of GAD67-GFP knock-in mice. The aim of this study was to investigate the presence of GABA system in upper airway tract. To observe GAD expression, mucous membranes of upper airway tract of adult male GAD67-GFP mice were observed by fluorescent microscopy. To examine GABA receptor expression, reverse transcription-polymerase chain reaction (RT-PCR) analysis of the mucous membranes was made. Strong GFP fluorescence was exclusively observed in the cells of olfactory epithelium. RT-PCR analysis showed that the olfactory epithelium express subunit mRNAs of all GABA receptor types; GABAA, GABAC and GABAB. From the results of this study, it was suggested that presence of GABA system in the olfactory epithelium of mammals.
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